Highly bright avidin-based affinity probes carrying multiple lanthanide chelates (2025)

Wirpsza, L., Pillai, S., Batish, M., Marras, S., Krasnoperov, L., & Mustaev, A. (2012). Highly bright avidin-based affinity probes carrying multiple lanthanide chelates. Journal of Photochemistry and Photobiology B: Biology, 116, 22-29. https://doi.org/10.1016/j.jphotobiol.2012.07.001

Wirpsza, Laura ; Pillai, Shyamala ; Batish, Mona et al. / Highly bright avidin-based affinity probes carrying multiple lanthanide chelates. In: Journal of Photochemistry and Photobiology B: Biology. 2012 ; Vol. 116. pp. 22-29.

@article{7a8293e1c8684a7b845a41c9ca03f1dd,

title = "Highly bright avidin-based affinity probes carrying multiple lanthanide chelates",

abstract = "Lanthanide ion luminescence has a long lifetime enabling highly sensitive detection in time-gated mode. The sensitivity can be further increased by using multiple luminescent labels attached to a carrier molecule, which can be conjugated to an object of interest. We found that up to 30 lanthanide chelates can be attached to avidin creating highly bright constructs. These constructs with Eu3+ chelates display synergistic effect that enhance the brightness of heavily modified samples, while the opposite effect was observed for Tb3+ chelates thereby significantly reducing their light emission. This undesirable quenching of Tb3+ luminophores was completely suppressed by the introduction of an aromatic spacer between the chelate and the protein attachment site. The estimated detection limit for the conjugates is in the 10-14-10-15 M range. We demonstrated a high sensitivity for the new probes by using them to label living cells of bacterial and mammalian origin.",

keywords = "Chelates, Labeling, Lanthanide, Luminescense, Multiple, Synthesis",

author = "Laura Wirpsza and Shyamala Pillai and Mona Batish and Salvatore Marras and Lev Krasnoperov and Arkady Mustaev",

note = "Funding Information: This study was supported by NIH Grant RO1 GM-307-17-21 to AM and NIH Grant RO1 MN-079197 for SM and MB.",

year = "2012",

month = nov,

day = "5",

doi = "10.1016/j.jphotobiol.2012.07.001",

language = "English (US)",

volume = "116",

pages = "22--29",

journal = "Journal of Photochemistry and Photobiology B: Biology",

issn = "1011-1344",

publisher = "Elsevier B.V.",

}

Wirpsza, L, Pillai, S, Batish, M, Marras, S, Krasnoperov, L & Mustaev, A 2012, 'Highly bright avidin-based affinity probes carrying multiple lanthanide chelates', Journal of Photochemistry and Photobiology B: Biology, vol. 116, pp. 22-29. https://doi.org/10.1016/j.jphotobiol.2012.07.001

Highly bright avidin-based affinity probes carrying multiple lanthanide chelates. / Wirpsza, Laura; Pillai, Shyamala; Batish, Mona et al.
In: Journal of Photochemistry and Photobiology B: Biology, Vol. 116, 05.11.2012, p. 22-29.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Highly bright avidin-based affinity probes carrying multiple lanthanide chelates

AU - Wirpsza, Laura

AU - Pillai, Shyamala

AU - Batish, Mona

AU - Marras, Salvatore

AU - Krasnoperov, Lev

AU - Mustaev, Arkady

N1 - Funding Information:This study was supported by NIH Grant RO1 GM-307-17-21 to AM and NIH Grant RO1 MN-079197 for SM and MB.

PY - 2012/11/5

Y1 - 2012/11/5

N2 - Lanthanide ion luminescence has a long lifetime enabling highly sensitive detection in time-gated mode. The sensitivity can be further increased by using multiple luminescent labels attached to a carrier molecule, which can be conjugated to an object of interest. We found that up to 30 lanthanide chelates can be attached to avidin creating highly bright constructs. These constructs with Eu3+ chelates display synergistic effect that enhance the brightness of heavily modified samples, while the opposite effect was observed for Tb3+ chelates thereby significantly reducing their light emission. This undesirable quenching of Tb3+ luminophores was completely suppressed by the introduction of an aromatic spacer between the chelate and the protein attachment site. The estimated detection limit for the conjugates is in the 10-14-10-15 M range. We demonstrated a high sensitivity for the new probes by using them to label living cells of bacterial and mammalian origin.

AB - Lanthanide ion luminescence has a long lifetime enabling highly sensitive detection in time-gated mode. The sensitivity can be further increased by using multiple luminescent labels attached to a carrier molecule, which can be conjugated to an object of interest. We found that up to 30 lanthanide chelates can be attached to avidin creating highly bright constructs. These constructs with Eu3+ chelates display synergistic effect that enhance the brightness of heavily modified samples, while the opposite effect was observed for Tb3+ chelates thereby significantly reducing their light emission. This undesirable quenching of Tb3+ luminophores was completely suppressed by the introduction of an aromatic spacer between the chelate and the protein attachment site. The estimated detection limit for the conjugates is in the 10-14-10-15 M range. We demonstrated a high sensitivity for the new probes by using them to label living cells of bacterial and mammalian origin.

KW - Chelates

KW - Labeling

KW - Lanthanide

KW - Luminescense

KW - Multiple

KW - Synthesis

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U2 - 10.1016/j.jphotobiol.2012.07.001

DO - 10.1016/j.jphotobiol.2012.07.001

M3 - Article

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AN - SCOPUS:84865478975

SN - 1011-1344

VL - 116

SP - 22

EP - 29

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

ER -

Wirpsza L, Pillai S, Batish M, Marras S, Krasnoperov L, Mustaev A. Highly bright avidin-based affinity probes carrying multiple lanthanide chelates. Journal of Photochemistry and Photobiology B: Biology. 2012 Nov 5;116:22-29. doi: 10.1016/j.jphotobiol.2012.07.001